Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,110,5990G→A100.0% 1.7 2G259G (GGC→GGTinsF‑1putative transposase for IS3
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (1/0):  total (2/0)

TTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCG  >  REL606/1110573‑1110622
                          |                       
tgattttCAAATTGTTCCGGACTGAGACCGTCtcac                >  1:4160900/4‑36 (MQ=17)
              ttCCGGACTGAGGCCGCCACACCAACTGTgtcgtcg  >  1:3590277/1‑36 (MQ=1)
                          |                       
TTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCG  >  REL606/1110573‑1110622

Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG