Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,289,962 Δ16 bp coding (96‑111/4554 nt) gltB glutamate synthase, large subunit

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3289962 3289977 16 49 [0] [0] 49 gltB glutamate synthase, large subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew annotation gene product
* ? REL606 = 32899610 (0.000)48 (0.880) 34/70 0.2 coding (95/4554 nt) gltB glutamate synthase, large subunit
?REL606 3289978 = 0 (0.000)coding (112/4554 nt) gltB glutamate synthase, large subunit

GGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTG  >  REL606/3289926‑3289996
                                   |                                   
ggggTTCCCGCAGAGCCTGGGGGAGGTTCACGatat                                     >  1:2609713/1‑36 (MQ=255)
 gggTTCCCGCAGAGCCTGGGGGAGGTTCACGATATc                                    <  1:4136029‑M1/36‑2 (MQ=255)
 gggTTCCCGCAGAGCCTGAGGGAGGTTCACGATATc                                    >  1:5136776‑M1/1‑35 (MQ=255)
   gTTCCCGCAGAGCCTGGGGGAGGTTCACGATATctt                                  >  1:3327311‑M1/1‑33 (MQ=255)
      cccGCAGAGCCTGGGGGAGGTTCACGATATcttgag                               <  1:321637‑M1/36‑7 (MQ=255)
       ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga                              >  1:2519051‑M1/1‑29 (MQ=255)
       ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga                              <  1:2435189‑M1/36‑8 (MQ=255)
       ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga                              >  1:728798‑M1/1‑29 (MQ=255)
       ccGCAGAGCCTGGGGGAGGTTCACGATATcttgaga                              <  1:1435538‑M1/36‑8 (MQ=255)
        cGCAGAGCCTGGGGGAGGTTCACGATATcttgagag                             >  1:4473088‑M1/1‑28 (MQ=255)
        cGCAGAGCCTGGGGGAGGTTCACGATATcttgagag                             >  1:5330195‑M1/1‑28 (MQ=255)
         gCAGAGCCTGGGGGAGGTTCACGATATcttgagagg                            >  1:2113304‑M1/1‑27 (MQ=255)
         gCAGAGCCTGGGGGAGGTTCACGATATcttgagagg                            >  1:4912198‑M1/1‑27 (MQ=255)
          cAGAGCCTGGGGGAGGTTCACGATATcttgagaggg                           >  1:5472267‑M1/1‑26 (MQ=255)
          cAGAGCCTGGGGGAGGTTCACGATATcttgagaggg                           >  1:7224075‑M1/1‑26 (MQ=255)
             agCCTGGGGGAGGTTCACGATATcttgagagggata                        >  1:7100640‑M1/1‑23 (MQ=255)
               ccTGGGGGAGGTTCACGATATcttgagaggtataac                      >  1:2719396‑M1/1‑21 (MQ=255)
               ccTGGGGGAGGTTCACGATATcttgagagggataac                      <  1:4509504‑M1/36‑16 (MQ=255)
               ccTGGGGGAGGTTCACGATATcttgagagggataac                      <  1:2617914‑M1/36‑16 (MQ=255)
                cTGGGGGGGGTTCACGATATcttgagagggataact                     <  1:6565020‑M1/36‑17 (MQ=255)
                cTGGGGGAGGTTCACGATATcttgagagggttaact                     >  1:3928093‑M1/1‑20 (MQ=255)
                cTGGGGGAGGTTCACGATATcttgagagggataact                     >  1:3070297‑M1/1‑20 (MQ=255)
                  gggggAGGTTCACGATATcttgagagggataactgt                   >  1:925597‑M1/1‑18 (MQ=255)
                  gggggAGGTTCACGATATcttgagagggataactgt                   >  1:1583709‑M1/1‑18 (MQ=255)
                  gggggAGGTTCACGATATcttgagagggataactgt                   <  1:1678757‑M1/36‑19 (MQ=255)
                   ggggAGGTTCACGATATcttgagagggataactgtg                  >  1:3231936‑M1/1‑17 (MQ=255)
                    gggAGGTTCACGATATcttgagagggataactgtgg                 >  1:7267416‑M1/1‑16 (MQ=255)
                    gggAGGTTCACGATATcttgagagggataactgtgg                 <  1:4806166‑M1/36‑21 (MQ=255)
                     ggAGGTTCACGATATcttgagagggataactgtggt                <  1:6348773‑M1/36‑22 (MQ=255)
                     ggAGGTTCACGATATcttgagagggataactgtggt                <  1:500979‑M1/36‑22 (MQ=255)
                      gAGGTTCACGATATcttgagagggataactgtggtt               <  1:3537557‑M1/36‑23 (MQ=255)
                      gAGGTTCACGATATcttgagagggataactgtggtt               >  1:4975305‑M1/1‑14 (MQ=255)
                      gAGGTTCACGATATcttgagagggataactgtggtt               <  1:6287136‑M1/36‑23 (MQ=255)
                      gAGATTCACGATATcttgagagggataactgtggtt               <  1:4049854‑M1/36‑23 (MQ=255)
                       aGGTTCACGATATcttgagagggataactgtggttt              <  1:5674725‑M1/36‑24 (MQ=255)
                       aGGTTCACGATATcttgagagggataactgtggttt              <  1:2623833‑M1/36‑24 (MQ=255)
                       aGGTTCACGATATcttgagagggataactgtggttt              >  1:280225‑M1/1‑13 (MQ=255)
                        ggTTCACGATATcttgagagggataactgtggtttc             <  1:4707617‑M1/36‑25 (MQ=255)
                         gTTCACGATATcttgagagggataactgtggtttcg            <  1:1818014‑M1/36‑26 (MQ=255)
                         gTTCACGATATcttgagagggataactgtggtttcg            >  1:3566879‑M1/1‑11 (MQ=255)
                           tCACGATATcttgagagggataactgtggtttcggc          <  1:6843675‑M1/36‑28 (MQ=255)
                            cACGATATcttgagagggataactgtggttttggcc         >  1:2533717‑M1/1‑8 (MQ=255)
                              cGATATcttgagagggataactgtggtttcggcctt       >  1:2887498‑M1/1‑6 (MQ=255)
                              cGATATcttgagagggataactgtggtttcggcctg       >  1:5849235‑M1/1‑6 (MQ=255)
                              cGATATcttgagagggataactgtggtttcggcctg       <  1:7070671‑M1/36‑31 (MQ=255)
                              cGATATcttgagagggataactgtggtttcggcctg       >  1:1604243‑M1/1‑6 (MQ=255)
                                  atcttgagagggataactgtggtttcggcctgatcg   <  1:3025852‑M1/36‑35 (MQ=255)
                                   tcttgagagggataactgtggtttcggcctgttcgc  >  1:2174694‑M1/1‑1 (MQ=255)
                                   tcttgagagggataactgtggtttcggcctgatcgc  <  1:7461580‑M1/36‑36 (MQ=255)
                                   |                                   
GGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTG  >  REL606/3289926‑3289996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG