LOCUS AY048742 2804 bp DNA circular SYN 11-SEP-2001 DEFINITION Template plasmid pKD3, complete sequence. ACCESSION AY048742 VERSION AY048742.1 GI:15554329 KEYWORDS . SOURCE Template plasmid pKD3 ORGANISM Template plasmid pKD3 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2804) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000) PUBMED 10829079 REFERENCE 2 (bases 1 to 2804) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE Direct Submission JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University, Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA FEATURES Location/Qualifiers source 1..2804 /organism="Template plasmid pKD3" /mol_type="genomic DNA" /db_xref="taxon:170491" misc_feature 31..50 /note="priming site 1" misc_feature 32..51 /note="priming site 3" misc_feature complement(51..84) /note="distal 34-nt of of natural FRT site" CDS complement(134..772) /note="Tn9" /codon_start=1 /transl_table=11 /product="chloramphenicol acetyl transferase" /protein_id="AAL02033.1" /db_xref="GI:15554330" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR" misc_feature 245..264 /note="common priming site c1" misc_feature 955..974 /note="common priming site c2" misc_feature complement(981..1022) /note="distal 42-nt of natural FRT site" misc_feature 1044..1063 /note="priming site 2" rep_origin 1047..1446 /note="R6K gamma replication origin" CDS complement(1516..2376) /note="bla; ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAL02034.1" /db_xref="GI:15554331" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" terminator 2523..2782 /note="rrnB transcription terminator" ORIGIN 1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta 61 tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg cgccttacgc 121 cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg acatggaagc 181 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 241 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 301 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 361 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 421 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 481 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 541 tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 601 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 661 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 721 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 781 ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat cttatttcat 841 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg 901 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt 961 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata 1021 ggaactaagg aggatattca tatggaccat ggctaattcc catgtcagcc gttaagtgtt 1081 cctgtgtcac tgaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct 1141 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt 1201 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt 1261 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc 1321 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat 1381 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgcgg 1441 atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 1561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 1621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 1681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 1741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 1801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 1861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 1921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 1981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 2041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 2101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 2161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 2221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 2281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 2341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 2401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 2461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat cgatggcccc 2521 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa 2581 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 2641 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga 2701 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc 2761 ctgacggatg gcctttttgc gtggccagtg ccaagcttgc atgc //