LOCUS X06403 4245 bp DNA circular SYN 14-NOV-2006 DEFINITION Cloning vector pACYC184. ACCESSION X06403 VERSION X06403.1 GI:58255 KEYWORDS chloramphenicol resistance; cloning vector; plasmid; tetracycline resistance. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 944; 946 to 4245) AUTHORS Rose,R.E. TITLE The nucleotide sequence of pACYC184 JOURNAL Nucleic Acids Res. 16 (1), 355 (1988) PUBMED 3340533 REFERENCE 2 (bases 945 to 945) AUTHORS Mok,Y.K., Clark,D.R., Kam,K.M. and Shaw,P.C. TITLE BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177 JOURNAL Nucleic Acids Res. 19 (9), 2321-2323 (1991) PUBMED 2041772 REFERENCE 3 AUTHORS Li,J.K. and Tu,J. TITLE Ssp RF1, a novel class-II restriction endonuclease from Synechococcus RF-1 recognizing 5'TT/CGAA-3' JOURNAL Nucleic Acids Res. 19 (17), 4770 (1991) PUBMED 1909785 REFERENCE 4 (bases 1 to 4244) AUTHORS Rose,R.E. TITLE Direct Submission JOURNAL Submitted (15-DEC-1987) Rose R.E., Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA COMMENT The sequence is circular, however the sequence begins with a unique EcoRI site. Bases 426-706, 1153-1583 and 2918 to 3735 were determined while the rest of the sequence was taken from the published sequence of pSC101, Tn9 and p15A. FEATURES Location/Qualifiers source 1..4245 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /plasmid="pACYC184" misc_feature 219..3805 /note="chloramphenicol resistance gene" misc_feature 443..583 /note="part of IS1 from Tn9" rep_origin 581..1493 /note="p15A origin of replication" misc_feature complement(585..3531) /note="Tn9 HaeII fragment" conflict 944..946 /citation=[1] /citation=[4] /replace="tc" misc_feature 1495..3276 /note="pSC101 fragment" misc_feature 1581..2771 /note="tetracycline resistance gene" ORIGIN 1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca 1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc 1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct 1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg 1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg 1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt 1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc 1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac 1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg 1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg 1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt 2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc 2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct 2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg 2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg 2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg 2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt 2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc 2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg 2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg 2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga 2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag 2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca 2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg 2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc 2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg 2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg 3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg 3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag 3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc 3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta 3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat 3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc 3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa 3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca 3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag 3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg 3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg 3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg 3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat 3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg 3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg 4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc 4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg //