50 Recent Changes in Lab Web retrieved at 13:30 (GMT)

Main.JeffreyBarrick

Barrick Lab Style Guide Writing a Scientific Paper Steps to Writing a Research Article (Tomorrow's Professor Mailing List Archive #1176) General Formatting ...
Denaturing Formaldehyde Gels for Verifying RNA size RNA can be sized correctly on an agarose gel. However, it needs to be denatured and then remain denatured during ...
Main.SimonDAlton

Isolation of Total RNA from Plant Material Plant tissues can be tough (roots), impenetrably waxy, or contain large amounts of RNase activity (leaves). Flash freezing ...
Back to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional ...
Main.JeffreyBarrick

Barrick Lab Members Prof. Jeffrey E. BarrickPrincipal Investigator (01/2011–present)@barricklab Dr. Simon D'AltonResearch Associate (09/2015 present ...
Main.TWikiGuest

Statistics for nop Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and ...
Keio and ASKA collections Dear internet, Our lab is unable to distribute the Keio/ASKA strains to other institutions. If you want to use these fantastic E. coli ...
Main.SimonDAlton

Barrick Lab :: Laboratory Protocols Creating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki. ...
JaredEller 14 Dec 2017 Vibrio natriegens has the fastest doubling time of any known organism and has the potential to shorten experimental timelines and increase bioproduction ...
Golden Gate Assembly This page serves as the main repository for everything Golden Gate Assembly related. This page links to a number of protocol pages that will ...
Back to Golden Gate Protocols Troubleshooting your Golden Gate Assemblies Tips Inserts should be screened for the presence of internal BsaI/BsmBI sites before ...
Back to Golden Gate Protocols Second Stage Assembly Second Stage assemblies are used to build plasmids out of multiple transcriptional units (Protocol found here ...
Back to Golden Gate Protocols Part Plasmid Assembly Once you have designed your part and either amplified with PCR or ordered the desired gBlock (as described here ...
iGEM Part Plasmid Assembly Below is the "Dr. Mishler recommends" Golden Gate Assembly reaction for FRI/iGEM students. Alternative Golden Gate Assembly Reaction ...
Back to Golden Gate Protocols Designing a new part Golden Gate Assembly Step 1: Creating dsDNA encoding the part for cloning Two variations on preparing a dsDNA ...
Golden Gate Assembly Background and Design Golden Gate cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously ...
Broad Host Range Toolkit The Broad host range toolkit (BTK) is a Golden Gate compatible toolkit of genetic parts designed for working with newly isolated, non model ...
Main.SimonDAlton

RNAseq Library Preparation Use RNA from RNASnap and Zymo coumn purification as outlined on wiki. rRNA Depletion Use the Ribozero (RZ) rRNA Removal Kit (Gram negative ...
Main.JeffreyBarrick

The University of Texas at Austin :: iGEM Team Donate to the UT Austin iGEM team to support travel, supplies, and research stipends for our excellent students! ...
Microplate Reader Quick Start 1 It's always best to start from single colony picks that had been grown overnight in liquid culture to saturation. 1 A typical ...
Main.JeffreyBarrick

The Lenski Long Term Evolution Experiment Arabinose Marker REL606 and REL607 (the ancestral strains of the E. coli long term evolution experiment) differ by a single ...
Main.JeffreyBarrick

Past Barrick Lab Members Xue Zhang Michael Hammerling Brian Renda Jordan Monk Craig Barnhart Aurko Dasgupta Lindsey Wolf Alvaro Rodriguez Neil Gottel Vinicio Reynoso ...
Media Amendment Stock Solutions Reagent Abb Stock Conc. Working Conc. Dilution Solvent MSDS Notes Ampicillin Amp 100 mg/mL ...
Main.DanielDeatherage

Instructions for how to analyze fluctuation test data using the command line. Please see this page for information regarding how to conduct a fluctuation analysis ...
Main.CamiloGomez

Commonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points ...
Working with Serratia marcescens Serratia marcescens is a gram negative pathogenic bacteria known for its distinctive red pigmentation. While it is not found in ...
Determining Phage Titer 1 Microwave flask of Top Agar (LB 0.8% agar) to melt. Allow to cool until handleable, then prepare 4 ml aliquots in small test tubes for ...
Phage Lysate Preparation Definitely works with T4 and T7, should work with most E. coli phages (although lysis times and final titers may differ) Reagents / ...
Absolute QPCR for quantification of plasmid copy number in E. coli This protocol is based on methods described in Lee et al (2006), to paper. Designing primers ...
Main.JuliePerreau

Barrick Lab :: Computational Protocols Command line Unix Commands Quick Reference Collection of useful Unix commands. SSH Public Key Authentication How ...
Gibson Assembly Background and Design Gibson Cloning is a technique of DNA construct assembly that allows one to join multiple linear segments into either one large ...
#TransformingElectroporation Transforming E. coli Cells by Electroporation This procedure uses the TOP10 Electrocomp E. coli cells (but is identical in any ...
General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol ...
Step 1: Creating dsDNA encoding the part for cloning Two variations on preparing a dsDNA fragment with the proper restriction sites and Golden Gate overhangs are provided ...
Autoclave Sterilization Overview Autoclaves heat their contents to 121°C, which is 21°C over the boiling point of water. To prevent the solutions from boiling over ...
Main.JeffreyBarrick

How to Create a Protocol Tips for Design You should generally organize a protocol to have sections that are relevant from this list: Supplies (materials, strains ...
Main.SimonDAlton

Quantitative real time PCR for differential gene expression The following is a comprehensive strategy for qPCR that works and is practically, mathematically and statistically ...
Main.PengGeng

Megaprimer whole plasmid cloning aka nop MEGAWHOP cloning aka Overlap Extension PCR cloning Adapted from Bryksin AV, Matsumura I. 2010. Overlap extension PCR Cloning ...
Main.DanielDeatherage

Fluctuation Tests This protocol is for doing a Luria Delbrück fluctuation test to measure the rate at which mutations occur that enable growth on selective agar. This ...
Main.JeffreyBarrick

Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab members Equal contributions ^corresponding author(s) 2017 72. Good ...
Main.DaciaLeon

Measuring Intracellular Reactive Oxygen Species (ROS) This procedure is commonly utilized to quantify ROS. For more information about limitations associated with this ...
Main.GabrielSuarez

ABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized ...
Main.GabrielSuarez

Growth Rates using "R studio" Overview Determining growth rates for many cultures or from plate reader data can be a daunting task. To automate this process, we ...
Dpn I digestion Purpose To digest (Adeno) methylated GATC sites. Useful for removing cell derived plasmid template from PCR samples. Use 1. Add 1µL of DpnI to ...
Main.JeffreyBarrick

Interested in working in the Barrick lab? We are always looking for outstanding undergraduate students, graduate students, and postdocs who are interested in experimental ...
"In plate" / solid medium transformation of Acinetobacter baylyi ADP1 The following protocol is for single colony "in situ" (in plate) transformation with minimal ...
Main.SimonDAlton

Contacts Campus Resources The following services may be able to help fix anything from a Faulty Freezer to a Questionable Qubit. Facilities. Have refrigerator and ...
SeanLeonard 14 Sep 2017
SeanLeonard 14 Sep 2017
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Topic revision: r2 - 28 Mar 2005 - 09:40:13 - Main.TWikiContributor