Commonly Used E. coli Strains

OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains.

The following table points out some important features to take into account when working with some these E. coli strains.

Strain Genotype Observations
E. coli DH5α F endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ( lacZYA-argF)U169, hsdR17(rKmK+), λ This strain is auxotrophic for thiamine ( thi-1) so that requires supplementation to be grown in minimal media such as DM. It has been observed that in minimum medium, even supplemented with thiamine (i.e. M9), this strain grows slowly compared to other strains such as BL21 and BW25113.
E. coli TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-  
E. coli BL21 F ompT gal dcm lon hsdSB(rBmB) [malB+]K-12S)  
E. coli BW25113

lacI+rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 rph-1
Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1

 

-- Main.CamiloGomez - 14 Dec 2017

 Barrick Lab  >  ProtocolList  >  Strain_side_notes

Topic revision: r1 - 14 Dec 2017 - 22:04:00 - Main.CamiloGomez
 
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