Commonly Used Plasmids

plasmid markers origin ref description
pCA24N CamR   Kitagawa2005 ASKA collection vector
pKD46 AmpR TS Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin grow at 30C! Low copy number.
pKD78 CamR TS   Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3 CamR   Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR     TOPO cloning kit vector
pACYC184 TetR, CamR   Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR   Link1997 Church vectors for creating deletions; note: Grow at 30 degrees Celsius
pRA300, pRA301, pRA302 SpecR   Akakura2002 Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in 80C freezer in both Welch and MBB.

plasmid strain
pACBSR TOP10
pKD3 BW25141
pKD13 BW2514
pKD32 BW2514
pKD46 BW25141
pCP20 BT340
pRA300 ?
pRA301 ?
pRA302 ?
pACYC184 ER2420

References

Akakura2002 Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978 Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000 Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):66406645.
Kitagawa2005 Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997 Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.
Topic attachments
I Attachment Action Size Date Who Comment
elsegbk pACYC184.gbk manage 7.7 K 03 Aug 2009 - 21:09 Main.JeffreyBarrick  
elsegbk pCA24N.gbk manage 9.9 K 29 May 2009 - 13:21 Main.JeffreyBarrick  
elsegbk pCR2.1.gbk manage 7.1 K 07 Mar 2012 - 21:11 Main.JeffreyBarrick  
elsegbk pKD13_Kan_template.gbk manage 7.6 K 29 May 2009 - 13:22 Main.JeffreyBarrick  
elsegbk pKD3_Cam_template.gbk manage 6.6 K 29 May 2009 - 13:22 Main.JeffreyBarrick  
elsegb pKD46.gb manage 16.4 K 20 Jan 2012 - 21:09 Main.BrianRenda  
txttxt pKOV.txt manage 10.6 K 21 Feb 2011 - 17:13 Main.JeffreyBarrick  
elsegbk pkd4.gbk manage 6.1 K 19 Dec 2011 - 22:29 Main.BrianRenda  
elsegbk pkd4_with_gfp_sites_marked.gbk manage 10.0 K 19 Dec 2011 - 22:35 Main.BrianRenda  
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Topic revision: r19 - 25 Mar 2013 - 18:43:30 - Main.DanielDeatherage
 
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