Commonly Used Plasmids

plasmid markers origin host ref description
pCA24N CamR       Kitagawa2005 ASKA collection vector
pKD46 AmpR TS ? Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78 CamR TS     Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3 CamR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR       TOPO cloning kit vector
pACYC184 TetR, CamR   ER2420 Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR     Link1997 Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR CamR p15A   Herring2003 Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302 SpecR     Akakura2002 Broad host range, promoterless lacZ map
pCP20     BT340    

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Topic attachments
I Attachment History Action Size Date Who Comment
Unknown file formatgbk pACYC184.gbk r1 manage 7.7 K 2009-08-03 - 21:09 JeffreyBarrick  
Unknown file formatgbk pCA24N.gbk r1 manage 9.9 K 2009-05-29 - 13:21 JeffreyBarrick  
Unknown file formatgbk pCR2.1.gbk r2 r1 manage 7.1 K 2012-03-07 - 21:11 JeffreyBarrick  
Unknown file formatgbk pKD13_Kan_template.gbk r1 manage 7.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgbk pKD3_Cam_template.gbk r1 manage 6.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgb pKD46.gb r1 manage 16.4 K 2012-01-20 - 21:09 BrianRenda  
Texttxt pKOV.txt r1 manage 10.6 K 2011-02-21 - 17:13 JeffreyBarrick  
Unknown file formatgbk pkd4.gbk r1 manage 6.1 K 2011-12-19 - 22:29 BrianRenda  
Unknown file formatgbk pkd4_with_gfp_sites_marked.gbk r1 manage 10.0 K 2011-12-19 - 22:35 BrianRenda  
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Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, DanielDeatherage, BrianRenda, AlvaroRodriguez
Topic revision: r20 - 2013-11-01 - 16:14:42 - Main.JeffreyBarrick
 
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