SOC: Super Optimal Broth with Glucose

Used for recovery of cells after electroporation or heat shock transformation.

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave. When solution has cooled to 50C (cool enough to touch with bare hands for a few seconds), add 40 ml of autoclaved 10% glucose.

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Topic revision: r5 - 11 Jan 2012 - 18:08:13 - Main.CraigBarnhart
 
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