SOB: Super Optimal Broth Plates

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4
16 g   Agar
1ml   Antifoam

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave.

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Topic revision: r1 - 11 Jan 2012 - 18:13:21 - Main.CraigBarnhart
 
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