---+++RCV Medium RCV is a complex media consisting of 3 different sub-components that must be prepared ahead of time if they are not already made. *Main recipe* | *1L* | *Component* | | 10 mL | 10% (NH<sub>4</sub>)<sub>2</sub> SO<sub>4 | | 40 mL | 10% DL-malate, pH 6.8 | | 50 mL | Super salts (see below) | | 15 mL | 0.64 KPO<sub>4</sub>, pH 6.8 (see below) | add 3/4 H<sub>2</sub>O before adding the KPO<sub>4</sub> <br> adjust pH to 6.8 if needed<br> if making plates add 16 g Agar per L *0.64M KPO<sub>4</sub>, pH 6.8* | *500mL* | *Component* | | 20 g | KH<sub>2</sub>PO<sub>4 | | 30 g | K<sub>2</sub>HPO<sub>4 | *Super salts* | *1L* | *Component* | | 40 mL | 1.0% EDTA | | 20 mL | 20% MgSO<sub>4</sub> 5H<sub>2</sub>O | | 20 mL | 7.5% CaCl<sub>2</sub> 2H<sub>2</sub>O | | 20 mL | Trace elements (see below) | | 48 mL | 0.5% FeSO<sub>4</sub> 7H<sub>2</sub>O | | 20 mL | 0.1% thiamine-HCl | *Trace Elements* | *250mL* | *Component* | | 0.3975 g | MnSO<sub>4</sub>H<sub>2</sub>O | | 0.7 g | H<sub>3</sub>BO<sub>3</sub> (Boric Acid) | | 0.01 g | Cu(NO<sub>3</sub>)<sub>2</sub> 3H<sub>2</sub>O | | 0.06 g | ZnSO<sub>4</sub> 7H<sub>2</sub>O | | 0.1875 g | NaMoO<sub>4</sub> 2H<sub>2</sub>O (Sodium molybdate VI dihydrate) |
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Topic revision: r4 - 2019-03-29 - JeffreyBarrick