RCV Medium

RCV is a complex media consisting of 3 different sub-componets that must be prepared ahead of time if they are not already made.

Main recipe

1L Component
10 mL 10% (NH4)2 SO4
40 mL 10% DL-malate, pH 6.8
50 mL Super salts (see below)
15 mL 0.64 KPO4, pH 6.8 (see below)

add 3/4 H2O before adding the KPO4
adjust pH to 6.8 if needed
if making plates add 16 g Agar per L

0.64M KPO4, pH 6.8

500mL Component
20 g KH2PO4
30 g K2HPO4

Super salts

1L Component
40 mL 1.0% EDTA
20 mL 20% MgSO4 5H2O
20 mL 7.5% CaCl2 2H2O
20 mL Trace elements (see below)
48 mL 0.5% FeSO4 7H2O
20 mL 0.1% thiamine-HCl

Trace Elements

250mL Component
0.3975 g MnSO4H2O
0.7 g H3BO3 (Boric Acid)
0.01 g Cu(NO3)2 3H2O
0.06 g ZnSO4 7H2O
0.1875 g NaMoO4 2H2O (Sodium molybdate VI dihydrate)

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Topic revision: r3 - 01 Aug 2012 - 19:26:56 - Main.CraigBarnhart
 
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