NZY+ Broth

1L Component
10 g NZ Amine (casein hydrolysate)
5 g Yeast Extract
5 g NaCl

Add dH2O to final volume. Adjust pH to 7.5 using NaOH (Requires ~350 Ál of 10 N). Autoclave. Then add.

1L Component
12.5 ml 1 M MgCl2
12.5 ml / 15.0 ml 1 M / 10% (w/v) MgSO4 FW = 120.3 g/mol
40 ml 10% (w/v) glucose

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Topic revision: r2 - 29 Jul 2011 - 14:18:12 - Main.CraigBarnhart
 
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