M9 Minimal
Make 5× M9 salts:
| 64 g |
Sodium phosphate (dibasic, 7 hydrate) Na2HPO4•H2O |
| 15 g |
Potassium phosphate (monobasic) KH2PO4 |
| 2.5 g |
Sodium chloride, NaCl |
| 5 g |
Ammonium chloride NH4Cl |
Add dH
2O to 1 liter. Autoclave.
Combine the following ingredients (all pre-sterilized):
| 200 ml |
5× M9 salts |
| 2 ml |
1M Magnesium sulfate MgSO4 |
| 0.1 ml |
1 M Calcium chloride CaCl2 |
| 40 ml |
10% (w/v) glucose (or other carbon source) |
Add
sterile dH
2O to 1 liter. Autoclave.
To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.