Barrick Lab :: ProtocolsRecipesM9

As with DM and MG media, make sure to autoclave the agar and phosphate separately.

For 1 liter of media:

1L	Component
6 g	Sodium phosphate, Na₂HPO_{4 (anhydrous)}
3 g	Potassium phosphate, KH₂PO₄
0.5 g	Sodium chloride, NaCl
1 g	Ammonium chloride, NH₄Cl

Add dH₂O to 500 mL

1L	Component
16 g	Agar
1 ml	Antifoam

Add dH₂O to 500 mL.

Autoclave media. Once the bottles are cooled to 55°C, mix the agar and M9 components.

Next, add the following sterile ingredients:

1L	Component
1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂
10 ml	10% (w/v) glucose (or other carbon source)

Contributors to this topic

DaciaLeon, CraigBarnhart

Topic revision: r3 - 2018-03-27 - 21:25:09 - Main.DaciaLeon