LB: Luria-Broth
Combine in a 2 L flask:
| 15 g |
Tryptone |
| 7.5 g |
Yeast Extract |
| 15 g |
NaCl |
| 24 g |
Agar |
| 1.5 ml |
5% Antifoam |
Add dH
2O to 1.5 L. Autoclave.
Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!
To make "soft" agar for phage studies, use only 7 g agar per liter.
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> ProtocolsMediaRecipes > ProtocolsRecipesLysogenyBrothAgar
Topic revision: r2 - 2011-07-26 - CraigBarnhart