LB: Luria-Bertani

Rich media used for routine culture of E. coli and other bacteria at high cell densities.

1L 5L Component
10 g 50 g Tryptone
5 g 25 g Yeast Extract
10 g 50 g NaCl

Add dH2O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.

Note: This recipe is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

Expected results: E. coli strains grow to 5×109 cells/ml final density in LB.

Variant: 0.1×LB is made with 1 g Tryptone, 0.5 g Yeast Extract, and 10 g NaCl per liter. (It is 0.1× in the nutrients, but not the salt!)

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Topic revision: r4 - 29 Jul 2011 - 14:07:30 - Main.CraigBarnhart
Lab.ProtocolsRecipesLuriaBertani moved from Lab.ProtocolsRecipesSalineLuriaBertani on 08 Mar 2011 - 14:19 by Main.JeffreyBarrick
 
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