---+++ DR: Defined minimal media for D. radiodurans | *250 ml* | *500 ml* | *Component* | | 50 ml | 100 ml | 5x M9 salts | | 250 ul | 500 ul | 5mM MnCl<sub>2 | | 250 ul | 500 ul | 0.8M MgCl<sub>2 | | 250 ul | 500 ul | 0.18 M CaCl<sub>2 | | 2.5 ml | 5 ml | Syringe filtered Vitamins Mix | | 500 ul | 1 ml | Syringe filtered Amino Acid mix (see below) | Add dH<sub>2</sub>O to final volume. <br> Note: All components are either pre-sterilized or syringe filtered so the only things that need to be autoclaved are the receiving bottle and the added water. To prepare the amino acids: <br> | *Amount* | *Amino Acid* | | 1 g | L-Cys | | 0.5 g | L-His | | 0.5 g | L-Met | Dissolve these amino acids in 40 ml of dH<sub>2</sub>O
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ProtocolsRecipesDR
Topic revision: r1 - 2011-07-29 - CraigBarnhart