---+++ 1416: 4-hydroxybenzoic acid medium (for JJ-1b, Bacillus sp.) | *1L* | *4L* | *Component* | | 4.25 g | 17.0 g | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> trihydrate | | 1.00 g | 4.00 g | Sodium Phosphate (monobasic) NaH<sub>2</sub>PO<sub>4</sub> monohydrate | | 2.00 g | 8.00 g | Ammonium chloride NH<sub>4</sub>Cl | | 0.20 g | 0.40 g | Magnesium Sulfate MgSO<sub>4</sub> heptahydrate | | 12.0 mg | 48.0 mg | Ferrous Sulfate FeSO<sub>4</sub> heptahydrate | | 3.00 mg | 12.0 mg | Manganese Sulfate MnSO<sub>4</sub> monohydrate | | 3.00 mg | 12.0 mg | Zinc Sulfate ZnSO<sub>4</sub> heptahydrate | | 1.00 mg | 4.00 mg | Cobalt (II) Sulfate CoSO<sub>4</sub> | | 0.10 g | 0.40 g | Nitrilotriacetic acid * | | 1.00 g | 4.00 g | 4-Hydroxybenzoic acid * | * Dissolve these components in water made alkaline with concentrated NaOH before adding other components. Adjust medium for final pH 7.2, +/- 0.2. Autoclave for at least 15 minutes. If plates needed, add 15.0 g of agar per liter to the above recipe.
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Topic revision: r1 - 2013-06-24 - CraigBarnhart