MOB: Mobility Media

This media is useful for enhancing the mobility of ADP1, which moves across the plate by "twitching".

1L Final [ ] Component
5 g 0.5% agarose
5 g 2.0% tryptone
2.5 g 10 mM NaCl

After pouring your gel, let it set overnight at room temperature. The next day, spot with 1uL of your overnight-grown culture, leave the plate to dry for 15 minutes at room temperature, wrap in Parafilm, invert, and incubate at 30C. You should see spreading over the next 24 hours.

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Topic revision: r2 - 10 Dec 2018 - 21:02:01 - Main.JeffreyBarrick
 
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