(r1) Barrick Lab :: ProtocolsCulturingArsenophonusNasoniae

---+ Working with _Arsenophonus nasoniae_

_Arsenophonus nasoniae_ is symbiont of the wasp, _Nasonia vitripennis_,gut microbiota that can be cultivated _in vitro_. The isolation and characterization of the particular strain used were carried out by Dr. Robert L. Gherna and are described in depth in [[http://www.microbiologyresearch.org/docserver/fulltext/ijsem/41/4/ijs-41-4-563.pdf?expires=1487884673&id=id&accname=sgid026149&checksum=3A244793D35672397E881B8D6CE2EA26][here]].

---+++ _In vitro_ culture conditions

---++++ Plate Growth
 _Arsenophonus nasoniae_ DSMZ Strain: 15247 (type strain) can be cultured at room temperature; although recommended culturing temperature found in literature is 28°C. It grows robustly on agar plates after 3-5 days on the following media: 
   * Brain Heart Infusion Agar

This particular strain doesn't show any antibiotic resistance to antibiotics at E. coli concentrations, so all standard lab concentrations can be used when making plates.

---++++ Liquid Media
 _Arsenophonus nasoniae_ can be cultivated in the following liquid media (same general culture conditions as above, with shaking): 
   * Brain Heart Infusion Media

-- Main.KateElston - 23 Feb 2017

Barrick Lab > ProtocolList > ProtocolsCulturingArsenophonusNasoniae

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KateElston

Topic revision: r1 - 2017-02-23 - 21:15:12 - Main.KateElston