Day 1: Plating the Mixed Population

  1. Find microsatellite-containing strains in the -80C freezer. They will be labeled with the appropriate microsatellite sequence located in the rhamnose operon (eg. "(CA)12"). See below for the list of microsatellites.

Day 2: Picking Colonies

Day 3: Passaging Colonies

Number Sequence Position Fluorescent Tag?
1 (CA)6 rhaT-rhaD  
2 (CA)12 rhaT-rhaD  
3 (TA)12 rhaT-rhaD  
4 (A)24 rhaT-rhaD  
5 (AGA)8 rhaT-rhaD  
6 (GGA)8 rhaT-rhaD  
7 (G)9 rhaT-rhaD  
8 (G)12 rhaT-rhaD  

-- Main.LindseyWolf - 18 May 2011

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Topic revision: r1 - 18 May 2011 - 19:15:10 - Main.LindseyWolf
 
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