breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6062,721,8310C→T 8.1% 25.8 ‑42.0 ‑0.0233S194F (TCT→TTT) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
*REL6064,557,6290T→G100.0% 21.9 27T53P (ACC→CCC) yjiWhypothetical protein
*REL6062,767,8830G→T 6.5% 20.7 ‑30.3 0.0242L106M (CTG→ATG) ygbEconserved inner membrane protein
*REL606864,0690T→G100.0% 12.7 17V184G (GTG→GGG) yliCpredicted peptide transporter subunit: membrane component of ABC superfamily
*REL6062,759,8560T→G 7.2% 11.5 ‑41.6 0.0260F254V (TTT→GTT) ygbMhypothetical protein
*REL6062,724,4230A→C 8.5% 8.7 ‑47.9 0.0263T173P (ACC→CCC) srlRDNA‑bindng transcriptional repressor
*REL6061,111,0950C→G100.0% 8.4 17G94A (GGC→GCC) insF‑1putative transposase for IS3
*REL606664,6870T→G 1.9% 7.7 ‑3.3 ‑0.0318intergenic (‑78/‑48)gltL/insJ‑2ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein
*REL6063,660,2300T→G100.0% 7.3 15T332P (ACC→CCC) xylAxylose isomerase
*REL6062,726,9991.→C 1.9% 7.0 ‑8.5 ‑0.0215coding (151/1515 nt)ygaAanaerobic nitric oxide reductase transcription regulator
*REL6062,732,3200G→C 4.9% 6.2 ‑14.9 ‑0.0222intergenic (‑35/+118)hypF/hydNcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
*REL6062,520,6811.→T 1.6% 6.1 ‑0.5 ‑0.2183intergenic (‑93/‑53)dapA/gcvRdihydrodipicolinate synthase/DNA‑binding transcriptional repressor, regulatory protein accessory to GcvA
*REL6062,766,9581.→C 1.5% 5.8 0.0 ‑0.2194coding (394/711 nt)ispD2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
*REL6062,757,2130A→T 4.9% 4.1 ‑17.3 ‑0.0241I7F (ATC→TTC) ygbKhypothetical protein
*REL6062,737,1120T→G 6.8% 3.2 ‑31.1 0.0247C421W (TGT→TGGascBcryptic 6‑phospho‑beta‑glucosidase
*REL6062,752,0540T→G 6.4% 2.7 ‑34.2 0.0270G34G (GGT→GGGmutSDNA mismatch repair protein
*REL6062,775,4090T→G100.0% 2.6 10D141A (GAC→GCC) insJ‑3IS150 hypothetical protein
*REL6062,724,1560G→C 3.4% 2.4 ‑14.5 0.0241A84P (GCT→CCT) srlRDNA‑bindng transcriptional repressor
*REL606714,0150G→A100.0% 2.2 1D18N (GAC→AAC) ybfOconserved protein, rhs‑like
*REL6062,847,2521.→A 1.0% 2.2 ‑1.5 ‑0.0209coding (3371/3543 nt)recBexonuclease V (RecBCD complex), beta subunit
*REL6061,271,3541.→G 1.9% 1.8 ‑1.5 ‑0.0159coding (645/1101 nt)chaAcalcium/sodium:proton antiporter
*REL6063,827,9281.→T 1.1% 1.7 0.0 ‑0.0183intergenic (‑145/‑161)ibpA/yidQheat shock chaperone/conserved outer membrane protein
*REL6062,775,4100C→G100.0% 1.1 9D141H (GAC→CAC) insJ‑3IS150 hypothetical protein
*REL6063,398,6941.→G100.0% 1.1 1coding (541/1185 nt)tufprotein chain elongation factor EF‑Tu (duplicate of tufB)
*REL6063,398,7000G→T100.0% 1.1 1L179M (CTG→ATG) tufprotein chain elongation factor EF‑Tu (duplicate of tufB)
*REL606554,3770T→G100.0% 1.0 8V846G (GTG→GGG) ECB_00512putative tail component of prophage
*REL6063,398,6931.→A100.0% 1.0 1coding (542/1185 nt)tufprotein chain elongation factor EF‑Tu (duplicate of tufB)
*REL6062,739,5410C→A 2.5% 0.9 ‑1.3 ‑0.2246A24A (GCG→GCThycFformate hydrogenlyase complex iron‑sulfur protein
*REL6062,750,8160A→C 4.4% 0.7 ‑30.1 0.0232Y540S (TAC→TCC) fhlADNA‑binding transcriptional activator
*REL6061,498,1150T→G100.0% 0.6 7V11G (GTG→GGG) ECB_01413conserved hypothetical protein
*REL606378,3661.→G 1.3% 0.3 ‑1.7 ‑0.0159coding (77/912 nt)rdgCrecombination associated protein
*REL6062,728,1861.→G 1.2% 0.3 ‑4.9 ‑0.0169coding (851/1440 nt)norVanaerobic nitric oxide reductase flavorubredoxin
*REL606554,5310T→G100.0% 0.2 2S897R (AGT→AGGECB_00512putative tail component of prophage
*REL606554,6170T→G100.0% 0.2 3V926G (GTG→GGG) ECB_00512putative tail component of prophage
*REL606573,2020T→G100.0% 0.2 2H96P (CAC→CCC) ECB_00530conserved hypothetical protein
*REL6061,111,2900C→G100.0% 0.2 2R29P (CGC→CCC) insF‑1putative transposase for IS3
*REL6062,775,8420T→A100.0% 0.1 3intergenic (‑12/+237)insJ‑3/cysHIS150 hypothetical protein/phosphoadenosine phosphosulfate reductase
*REL606429,7190G→A100.0% ‑0.1 1L357L (CTG→TTG) insL‑2putative transposase insL for insertion sequence IS186
*REL606430,0740G→T100.0% ‑0.1 1A238A (GCC→GCAinsL‑2putative transposase insL for insertion sequence IS186
*REL606430,0750G→T100.0% ‑0.1 1A238D (GCC→GAC) insL‑2putative transposase insL for insertion sequence IS186
*REL606557,7890C→A100.0% ‑0.1 1C147* (TGC→TGAECB_00515conserved hypothetical protein