Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL606430,0750G→T100.0% ‑0.1 1A238D (GCC→GAC) insL‑2putative transposase insL for insertion sequence IS186
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (0/0):  total (1/0)

GCAATGAGACGTGCCGGAAAGGGAGCTCCGGCTTT  >  REL606/430045‑430079
                              |    
gCAATGAGACGTGCCGGAAAGGGAGCTCCTTCttt  >  1:6443221/1‑35 (MQ=1)
                              |    
GCAATGAGACGTGCCGGAAAGGGAGCTCCGGCTTT  >  REL606/430045‑430079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.