New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 294203 =75 (0.730)12 (0.160) 3/36 NT 18.3% intergenic (+473/‑54) ykgD/ykgE predicted DNA‑binding transcriptional regulator/predicted oxidoreductase
?REL606 836230 = 51 (0.660)coding (1876/2226 nt) ybiO predicted mechanosensitive channel

Only 100 of 179 total aligned reads displayed.

AAAAGAACGCCATATTAATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG  >  REL606/294169‑294237
                                  |                                  
cccacctttgctaactttgtccgcggcattggttc                                    >  1:16055946‑M1/35‑35 (MQ=255)
accacctttgctaactttgtccgcggcattggttc                                    <  1:13149231‑M1/1‑1 (MQ=255)
accacctttgctaactttgtccgcggcattggttc                                    >  1:5601566‑M1/35‑35 (MQ=255)
accacctttgctaactttgtccgcggcattggttc                                    >  1:14797231‑M1/35‑35 (MQ=255)
aaaaGAACGCCATATTAATTGATGATTGATccccc                                    >  1:2293901/1‑35 (MQ=255)
aaaaGAACGCCATATTAATTGATGATTGATccccc                                    <  1:3241064/35‑1 (MQ=255)
 ccacctttgctaactttgtccgcggcattggttCg                                   <  1:4083320‑M1/2‑1 (MQ=255)
 ccacctttgctaactttgtccgcggcattggttCg                                   <  1:12864899‑M1/2‑1 (MQ=255)
 ccacctttgctaactttgtccgcggcattggttCg                                   <  1:12239134‑M1/2‑1 (MQ=255)
 ccacctttgctaactttgtccgcggcattggttCg                                   <  1:2691723‑M1/2‑1 (MQ=255)
 ccacctttgctaactttgtccgcggcattggttCg                                   <  1:6028298‑M1/2‑1 (MQ=255)
 aaaGAACGCCATATTAATTGATGATTGATCCCCCg                                   <  1:14112681/35‑1 (MQ=255)
 aaaGAACGCCATATTAATTGATGATTGATCCCCCg                                   <  1:249422/35‑1 (MQ=255)
 aaaGAACGCCATATTAATTGATGATTGATCCCCCg                                   >  1:1921599/1‑35 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  >  1:14176664‑M1/33‑35 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  >  1:5363517‑M1/33‑35 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  >  1:7679221‑M1/33‑35 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  <  1:16583532‑M1/3‑1 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  >  1:2903152‑M1/33‑35 (MQ=255)
  cacctttgctaactttgtccgcggcattggttCgg                                  >  1:2693049‑M1/33‑35 (MQ=255)
  aaGAACGCCATATTAATTGATGATTGATCCCCCgg                                  >  1:1710879/1‑35 (MQ=255)
  aaGAACGCCATATTAATTGATGATTGATCCCCCgg                                  <  1:11288845/35‑1 (MQ=255)
   acctttgctaactttgtccgcggcattggttCGGt                                 <  1:16334344‑M1/4‑1 (MQ=255)
   acctttgctaactttgtccgcggcattggttCGGt                                 >  1:12163694‑M1/32‑35 (MQ=255)
   aGAACGCCATATTAATTGATGATTGATCCCCCGGt                                 >  1:18836887/1‑35 (MQ=255)
   aGAACGCCATATTAATTGATGATTGATCCCCCGGt                                 >  1:17922348/1‑35 (MQ=255)
    cctttgctaactttgtccgcggcattggttCGGTg                                >  1:15689662‑M1/31‑35 (MQ=255)
    cctttgctaactttgtccgcggcattggttCGGTg                                >  1:18243061‑M1/31‑35 (MQ=255)
    cctttgctaactttgtccgcggcattggttCGGTg                                >  1:11707909‑M1/31‑35 (MQ=255)
    catttgctaactttgtccgcggcattggttCGGTg                                >  1:12494036‑M1/31‑35 (MQ=255)
     ctttgctaactttgtccgcggcattggttCGGTgg                               <  1:7404513‑M1/6‑1 (MQ=255)
     ctttgctaactttgtccgcggcattggttCGGTgg                               <  1:9129269‑M1/6‑1 (MQ=255)
     ctttgctaactttgtccgcggcattggttCGGTgg                               >  1:8767018‑M1/30‑35 (MQ=255)
     ctttgctaactttgtccgcggcattggttCGGTgg                               <  1:16255440‑M1/6‑1 (MQ=255)
     ctttgctaactttgtccgcggcattggttCGGTgg                               <  1:1097208‑M1/6‑1 (MQ=255)
      tttgctaactttgtccgcggcattggttCGGTggg                              <  1:3542413‑M1/7‑1 (MQ=255)
      tttgctaactttgtccgcggcattggttCGGTggg                              <  1:13514938‑M1/7‑1 (MQ=255)
      tttgctaactttgtccgcggcattggttCGGGggg                              <  1:8393756‑M1/7‑1 (MQ=255)
      aCGCCATATTAATTGATGATTGATCCCCCGGTggg                              <  1:6501346/35‑1 (MQ=255)
      aCGCCATATTAATTGATGATTGATCCCCCGGTggg                              <  1:8148488/35‑1 (MQ=255)
       cGCCATATTAATTGATGATTGATCCCCCGGTgggg                             <  1:6781166/35‑1 (MQ=255)
        tgctaactttgtccgcggcattggttCGGTggggg                            <  1:6902590‑M1/9‑1 (MQ=255)
        tgctaactttgtccgcggcattggttCGGTggggg                            <  1:844556‑M1/9‑1 (MQ=255)
        tgctaactttgtccgcggcattggttCGGTggggg                            <  1:1149800‑M1/9‑1 (MQ=255)
        gCCATATTAATTGATGATTGATCCCCCTGTggggg                            >  1:13825565/1‑35 (MQ=255)
        gCCATATTAATTGATGATTGATCCCCCGGTggggt                            >  1:5402418/1‑34 (MQ=255)
        gCCATATTAATTGATGATTGATCCCCCGGTggggg                            >  1:2396582/1‑35 (MQ=255)
         gctaactttgtccgcggcattggttCGGTgggggg                           <  1:6675886‑M1/10‑1 (MQ=255)
         ccATATTAATTGATGATTGATCCCCCGGTgggggg                           >  1:5259118/1‑35 (MQ=255)
         ccATATTAATTGATGATTGATCCCCCGGTgggggg                           >  1:4864808/1‑35 (MQ=255)
          cATATTAATTGATGATTGATCCCCCGGTggggggg                          >  1:12019805/1‑35 (MQ=255)
          cATATTAATTGATGATTGATCCCCCGGTggggggg                          >  1:1230857/1‑35 (MQ=255)
          cATATTAATTGATGATTGATCCCCCGGTggggggg                          >  1:20047440/1‑35 (MQ=255)
          cATATTAATTGATGATTGATCCCCCGGTggggggg                          >  1:9188637/1‑35 (MQ=255)
           taactttgtccgcggcattggttCggtgggggcgg                         <  1:12676819‑M1/12‑4 (MQ=255)
           taactttgtccgcggcattggttCGGTGGGGGGGa                         <  1:796267‑M1/12‑1 (MQ=255)
           taactttgtccgcggcattggttCGGGGGGGGGGa                         <  1:13430588‑M1/12‑1 (MQ=255)
           atatTAATTGATGATTGATCCCCCGGTGGGTGGGa                         >  1:18726621/1‑35 (MQ=255)
           atatTAATTGATGATTGATCCCCCGGTGGGGGGGa                         <  1:2562852/35‑1 (MQ=255)
           atatTAATTGATGATTGATCCCCCGGTGGGGGGGa                         >  1:6787733/1‑35 (MQ=255)
            tatTAATTGATGATTGATCCCCCGGTGGGGGGGaa                        >  1:6568438/1‑35 (MQ=255)
             atTAATTGATGATTGATCCCCCGGTGGGGGGGaaa                       >  1:20176347/1‑35 (MQ=255)
             atTAATTGATGATTGATCCCCCGGTGGGGGGGaaa                       <  1:8067574/35‑1 (MQ=255)
             actttgtccgcggcattggttCGGTGGGGGGgaat                       <  1:3143252‑M1/14‑2 (MQ=255)
             actttgtccgcggcattggttCGGTGGGGGGGaaa                       <  1:9641579‑M1/14‑1 (MQ=255)
             actttgtccgcggcattggttCGGTGGGGGGCaaa                       <  1:2408103‑M1/14‑1 (MQ=255)
              ttAATTGATGATTGATCCCCCGGTGGGGGGGAAAc                      >  1:16024632/1‑35 (MQ=255)
              ctttgtccgcggcattggttCGGTGGGGGGgaaat                      <  1:19058345‑M1/15‑2 (MQ=255)
               tAATTGATGATTGATCCCCCGGTGGGGGGGAAACa                     >  1:14051302/1‑35 (MQ=255)
               tAATTGATGATTGATCCCCCGGTGGGGGGGAAACa                     <  1:5487192/35‑1 (MQ=255)
                ttgtccgcggcattggttCGGTGGGGgggaaatat                    <  1:19425606‑M1/17‑4 (MQ=255)
                aaTTGATGATTGATCCCCCGGTGGGGGGGAAACAt                    <  1:2190750/35‑1 (MQ=255)
                aaTTGATGATTGATCCCCCGGTGGGGGGGAAACAt                    <  1:13737852/35‑1 (MQ=255)
                 aTTGATGATTGATCCCCCGGTGGGGGGGAAACATn                   <  1:1370562/35‑1 (MQ=255)
                 aTTGATGATTGATCCCCCGGTGGGGGGGAAACATc                   >  1:14271099/1‑35 (MQ=255)
                  ttGATGATTGATCCCCCGGTGGGGGGGAAACATCg                  <  1:5218778/35‑1 (MQ=255)
                  ttGATGATTGATCCCACGGTGGGGGGGAAACATCg                  <  1:12081910/35‑1 (MQ=255)
                   tgatgaTTGATCCCCCGGTGGGGGGGAAACATctg                 >  1:17516254/1‑33 (MQ=255)
                   tgatgaTTGATCCCCCGGTGGGGGGGAAACATCgg                 >  1:10433199/1‑35 (MQ=255)
                      tgattgatCCCCCGGTGGGTGGGAAACATCTGGCa              >  1:4830428/1‑35 (MQ=38)
                      tgattgatCCCCCGGTGGGGGGGAAACATCGGGCa              >  1:14605882/1‑35 (MQ=255)
                       gattgatCCCCCGGTGGGGGGGAAACATCGGGCAt             >  1:15835455/1‑35 (MQ=255)
                       gattgatCCCCCGGTGGGGGGGAAACATCGGGCAt             >  1:19374107/1‑35 (MQ=255)
                        attgatCCCCCGGTGGGGGGGAAACATCGGGCATa            <  1:3561422/35‑1 (MQ=255)
                         ttgatCCCCCGGTGGGGGGGAAACATCGGGCATaa           >  1:9173329/1‑35 (MQ=255)
                          tgatCCCCCGGTGGGGGGGAAACATCGGGCATaaa          >  1:6324814/1‑35 (MQ=255)
                          tgatCCCCCGGTGGGGGGGAAACATCGGGCATaaa          >  1:4278782/1‑35 (MQ=255)
                          tgatCCCCCGGTGGGGGGGAAACATCGGGCATaaa          >  1:7069759/1‑35 (MQ=255)
                           gatCCCCCGGTGGGTGGGAAACATCGGGCATAAAt         >  1:12780822/1‑35 (MQ=255)
                           gatCCCCCGGTGGGGTGGAAACATCGGGCATAAAt         >  1:17061227/1‑35 (MQ=255)
                            atCCCCCGGTGGGGGGGAAACATCGGGCATAAATg        >  1:16788897/1‑35 (MQ=255)
                             tCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg       <  1:17715432/35‑1 (MQ=255)
                              cccccGGTGGGGGGGAAACATCGGGCATAAATggg      >  1:18644054/1‑35 (MQ=255)
                                cccGGTGGGGGGGAAACATCGGGCATAAATGGGCa    >  1:4633642/1‑35 (MQ=255)
                                 ccGGTGGGGGGGAAACATCGGGCATAAATGGGCAt   <  1:14723052/35‑1 (MQ=255)
                                 ccGGTGGGGGGGAAACATCGGGCATAAATGGGCAt   <  1:15125193/35‑1 (MQ=255)
                                 ccGGTGGGGGGGAAACATCGGGCATAAATGGGCAt   <  1:16888426/35‑1 (MQ=255)
                                  CGGTGGGGGGGAAACATCGGGCATAAATGGGCATg  <  1:13957612‑M1/35‑1 (MQ=255)
                                  CGGTGGGGGGGAAACATCGGGCATAAATGGGCATg  <  1:11533299‑M1/35‑1 (MQ=255)
                                  CGGTGGGGGGGAAACATCGGGCATAAATGGGCATg  <  1:11116027‑M1/35‑1 (MQ=255)
                                  |                                  
AAAAGAACGCCATATTAATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATG  >  REL606/294169‑294237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.