Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6062,737,1120T→G 6.8% 3.2 ‑31.1 0.0247C421W (TGT→TGGascBcryptic 6‑phospho‑beta‑glucosidase
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands.
Rejected: Prediction has biased read strand distribution supporting polymorphism.
Reads supporting (aligned to +/- strand):  new base (25/0):  ref base (55/167):  total (80/167)
Fisher's exact test strand distribution p-value = 3.02e-14
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGCCTCTACGGGTGAAATGA  >  REL606/2737078‑2737146
                                  |                                  
 cATTCCGCTGATGGGCTACACCACATGGGGCTGTa                                   <  1:10714029/35‑1 (MQ=255)
  aTTCCGCTGATGGGCTACACCACATGGGGCTGTAt                                  <  1:18991819/35‑1 (MQ=255)
  aTTCCGCTGATGGGCTACACCACATGGGGCTGTAt                                  >  1:7888893/1‑35 (MQ=255)
  aTTCCGCTGATGGGCTACACCACATGGGGCTGGAt                                  >  1:16783930/1‑35 (MQ=255)
  aTTCCGCTGATGGGCTACACCACATGGGGCTGGAt                                  >  1:9325816/1‑35 (MQ=255)
   ttCCGCTGATGGGCTACACCACATGGGGCTGTAtt                                 <  1:17092076/35‑1 (MQ=255)
   ttCCGCTGATGGGCTACACCACATGGGGCTGTAtt                                 <  1:17422373/35‑1 (MQ=255)
   ttCCGCTGATGGGCTACACCACATGGGGCTGTAtt                                 <  1:3996381/35‑1 (MQ=255)
    tCCGCTGATGGGCTACACCACATGGGGCTGTATTg                                <  1:20162444/35‑1 (MQ=255)
    tCCGCTGATGGGCTACACCACATGGGGCTGTATTg                                <  1:11647880/35‑1 (MQ=255)
    tCCGCTGATGGGCTACACCACATGGGGCTGTATTg                                <  1:11963369/35‑1 (MQ=255)
    tCCGCTGATGGGCTACACCACATGGGGCTGGATTg                                >  1:15101686/1‑35 (MQ=255)
     ccGCTGATGGGCTACACCACATGGGGCTGTATTGa                               <  1:17957536/35‑1 (MQ=255)
     ccGCTGATGGGCTACACCACATGGGGCTGGAttta                               >  1:8294008/1‑33 (MQ=25)
     ccGCTGATGGGCTACACCACATGGGGCTGGATTGa                               >  1:12642763/1‑35 (MQ=255)
     ccGCTGATGGGCTACACCACATGGGGCTGGATTGa                               >  1:18512439/1‑35 (MQ=255)
     ccGCTGATGGGCTACACCACATGGGGCTGGATTGa                               >  1:19766744/1‑35 (MQ=255)
     ccGATGATGGGCTACACCACATGGGGCTGTATTGa                               <  1:6624994/35‑1 (MQ=255)
      cGCTGATGGGCTACACCACATGGGGCTGTATTggt                              >  1:12735122/1‑33 (MQ=255)
      cGCTGATGGGCTACACCACATGGGGCTGTATTGAt                              <  1:14128527/35‑1 (MQ=255)
      cGCTGATGGGCTACACCACATGGGGCTGTATTGAt                              >  1:1099324/1‑35 (MQ=255)
      cGCTGATGGGCTACACCACATGGGGCTGTATTGAt                              <  1:10348423/35‑1 (MQ=255)
      cGCTGATGGGCTACACCACATGGGGCTGGATTGAt                              >  1:13489232/1‑35 (MQ=255)
       gCTGATGGGCTACACCACATGGGGCTGTATTGAtt                             >  1:586708/1‑35 (MQ=255)
       gCTGATGGGCTACACCACATGGGGCTGTATTGAtt                             <  1:18078008/35‑1 (MQ=255)
       gCTGATGGGCTACACCACATGGGGCTGGATTTAtt                             >  1:18592017/1‑35 (MQ=25)
       gCTGATGGGCTACACCACATGGGGCTGGATTGAtt                             >  1:14893399/1‑35 (MQ=255)
        cTGATGGGCTACACCACATGGGGCTGTATTGTttt                            >  1:14469454/1‑35 (MQ=255)
        cTGATGGGCTACACCACATGGGGCTGTATTGAttt                            >  1:13746779/1‑35 (MQ=255)
        cTGATGGGCTACACCACATGGGGCTGGGTTTAttt                            >  1:8716625/1‑35 (MQ=21)
        cTGATGGGCTACACCACATGGGGCTGGCTTGAttt                            >  1:352904/1‑35 (MQ=25)
        cTGATGGGCTACACCACATGGGGCTGGATTGAttt                            >  1:15215197/1‑35 (MQ=255)
        cTGATGGGCTACACCACATGGGGCTGGATTGAttt                            >  1:4578349/1‑35 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGTATTGATTTa                           <  1:15250651/35‑1 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGTATTGATTTa                           <  1:14683689/35‑1 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGTATTGATTTa                           <  1:18164270/35‑1 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGTATTGATTTa                           >  1:578090/1‑35 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGTATTGATTTa                           <  1:11418627/35‑1 (MQ=255)
         tGATGGGCTACACCACATGGGGCTGGATTGATTTa                           >  1:9848331/1‑35 (MQ=255)
          gATGGTCTACACCACATGGGGCTGGATTGATTTAg                          >  1:10096580/1‑35 (MQ=37)
          gATGGGCTACACCACATGGGGCTGGATTGATTTAg                          >  1:6075205/1‑35 (MQ=255)
           aTGGGCTACACCACATGGGGCTGTATTGATTTAGt                         <  1:2044340/35‑1 (MQ=255)
           aTGGGCTACACCACATGGGGCTGGATTTATTTAGt                         >  1:7557745/1‑35 (MQ=37)
            tGGGCTACACCACATGGGGCTGTATTGATTTAGtt                        <  1:15039542/35‑1 (MQ=255)
            tGGGCTACACCACATGGGGCTGTATTGATTTAGtt                        <  1:18359334/35‑1 (MQ=255)
            tGGGCTACACCACATGGGGCTGTATTGATTTAGtt                        <  1:11235950/35‑1 (MQ=255)
            tGGGCTACACCACATGGGGCTGTATTGATTTAGtt                        <  1:9696197/35‑1 (MQ=255)
             gggCTACACCACATGGGGCTGTATTGATTTAGttt                       <  1:10948066/35‑1 (MQ=255)
             gggCTACACCACATGGGGCTGGATTGATTTTGttt                       >  1:3108840/1‑35 (MQ=25)
             gggCTACACCACATGGGGCTGGATTGATTTAGttt                       >  1:19565477/1‑35 (MQ=255)
              ggCTACACCACATGGGGCTGTATTGATTTAGTTTc                      <  1:8654594/35‑1 (MQ=255)
              ggCTACACCACATGGGGCTGTATTGATTTAGTTTc                      <  1:13868656/35‑1 (MQ=255)
               gCTACACCACATGGGGCTGGATTGATTTNNtntnn                     >  1:2597176/1‑33 (MQ=25)
               gCTACACCACATGGGGCAGTATTGATTTAGTTTcc                     >  1:5394228/1‑35 (MQ=255)
                cTACACCACATGGGGCTGTATTGATTTAGTTTCCg                    <  1:4443703/35‑1 (MQ=255)
                cTACACCACATGGGGCTGTATTGATTTAGTTTCCg                    <  1:1338081/35‑1 (MQ=255)
                cTACACCACATGGGGCTGTATTGATTTAGTTTCCg                    >  1:15710147/1‑35 (MQ=255)
                  acacCACATGGGGCTGTATTGATTTAGTTTCCGcc                  <  1:18886821/35‑1 (MQ=255)
                  acacCACATGGGGCTGTATTGATTTAGTTTCCGcc                  <  1:18702465/35‑1 (MQ=255)
                  acacCACATGGGGCTGTATTGATTTAGTTTCCGcc                  <  1:7127132/35‑1 (MQ=255)
                   caccacATGGGGCTGTATTGATTTAGTTTCCGCct                 <  1:17203863/35‑1 (MQ=255)
                   caccacATGGGGCTGTATTGATTTAGTTTCCGCct                 <  1:8342283/35‑1 (MQ=255)
                   caccacATGGGGCTGTATTGATTTAGTTTCCGCct                 <  1:16040463/35‑1 (MQ=255)
                    accacATGGGGCTGTATTGATTTAGTTTCCGCctc                <  1:8189430/35‑1 (MQ=255)
                     ccacATGGGGCTGTATTGATTTAGTTTCCGCctct               <  1:5678705/35‑1 (MQ=255)
                     ccacATGGGGCTGTATTGATTTAGTTTCCGCctct               <  1:15461557/35‑1 (MQ=255)
                     ccacATGGGGCTGGATTGATTTAGTTTCCGCctct               >  1:8786370/1‑35 (MQ=255)
                      cacaTGGGGCTGTATTGATTTAGTTTCCGCCTCTa              <  1:6394854/35‑1 (MQ=255)
                      cacaTGGGGCTGTATTGATTTAGTTTCCGCCTCTa              <  1:15373909/35‑1 (MQ=255)
                         aTGGGGCTGTATTGATTTAGTTTCCGCCTCTACgg           >  1:12230845/1‑35 (MQ=255)
                         aTGGGGCTGTATTGATTTAGTTTCCGCCTCTACgg           <  1:13070438/35‑1 (MQ=255)
                         aTGGGGCTGTATTGATTTAGTTTCCGCCTCTACgg           <  1:15630279/35‑1 (MQ=255)
                         aTGGGGCTGTATTGATTTAGTTTCCGCCTCTACgg           <  1:2168179/35‑1 (MQ=255)
                         aTGGGGCTGTATTGATTTAGTTTCCGCCTCTACgg           >  1:14411492/1‑35 (MQ=255)
                          tGGGGCTGTATTGATTTAGTTTCCGCCTCTACggg          <  1:3781160/35‑1 (MQ=255)
                            gggCTGTATTGATTTAGTTTCCGCCTCTACGGGTg        <  1:7812252/35‑1 (MQ=255)
                            gggCTGTATTGATTTAGTTTCCGCCTCTACGGGTg        >  1:17722049/1‑35 (MQ=255)
                            gggCTGTATTGATTTAGTTTCCGCCTCTACGGGTg        <  1:16616194/35‑1 (MQ=255)
                             ggCTGTATTGATTTAGTTTCCGCCTCTACGGGTGa       <  1:12020806/35‑1 (MQ=255)
                              gCTGTATTGATTTAGTTTCCGCCTCTACGGGTGaa      >  1:12851091/1‑35 (MQ=255)
                              gCTGTATTGATTTAGTTTCCGCCTCTACGGGTGaa      >  1:486725/1‑35 (MQ=255)
                               cTGTATTGATTTAGTTTCCGCCTCTACGGGTGaaa     <  1:4178846/35‑1 (MQ=255)
                                tGTATTGATTTAGTTTCCGCCTCTACGGGTGAAAt    <  1:2010921/35‑1 (MQ=255)
                                tGTATTGATTTAGTTTCCGCCTCTACGGGTGAAAt    >  1:7241594/1‑35 (MQ=255)
                                tGTATTGATTTAGTTTCCGCCTCTACGGGTGAAAt    >  1:19204842/1‑35 (MQ=255)
                                tGTATTGATTTAGTTTCCGCCTCTACGGGTGAAAt    <  1:9077246/35‑1 (MQ=255)
                                tGTATTGATTTAGTTTCCGCCTCTACGGGTGAAAt    >  1:856177/1‑35 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGGAATg   >  1:3254247/1‑35 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGAAATg   >  1:5290059/1‑35 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGAAATg   <  1:11903651/35‑1 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGAAATg   >  1:15797618/1‑35 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGAAATg   <  1:17693155/35‑1 (MQ=255)
                                 gTATTGATTTAGTTTCCGCCTCTACGGGTGAAATg   <  1:11008772/35‑1 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  <  1:1636988/35‑1 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  <  1:2271192/35‑1 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  <  1:233339/35‑1 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  <  1:4659685/35‑1 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  >  1:555662/1‑35 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  >  1:74057/1‑35 (MQ=255)
                                  tATTGATTTAGTTTCCGCCTCTACGGGTGAAATGa  <  1:6950795/35‑1 (MQ=255)
                                  |                                  
GCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGCCTCTACGGGTGAAATGA  >  REL606/2737078‑2737146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.