Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6062,759,8560T→G 7.2% 11.5 ‑41.6 0.0260F254V (TTT→GTT) ygbMhypothetical protein
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands.
Rejected: Prediction has biased read strand distribution supporting polymorphism.
Reads supporting (aligned to +/- strand):  new base (24/0):  ref base (32/204):  total (56/204)
Fisher's exact test strand distribution p-value = 8.87e-19
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

ACCTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAAT  >  REL606/2759822‑2759890
                                   |                                  
 ccTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGtt                                   <  1:7436830/35‑1 (MQ=255)
  cTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGttt                                  <  1:15033257/35‑1 (MQ=255)
  cTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGttt                                  <  1:10378677/35‑1 (MQ=255)
  cTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGgtt                                  >  1:16447581/1‑35 (MQ=255)
  cTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGgtt                                  >  1:7126802/1‑35 (MQ=255)
  cTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGgtt                                  >  1:11578331/1‑35 (MQ=255)
   tcttGGCCTCACCGAAGAAGGGCTTGGCTGGTTTg                                 <  1:15697148/32‑1 (MQ=255)
   tCGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTg                                 <  1:413914/35‑1 (MQ=255)
   tCGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTg                                 <  1:4832346/35‑1 (MQ=255)
   tCGTGGCCTCACCGAAGAAGGGCTTGGCTGGGTTg                                 >  1:1467673/1‑35 (MQ=255)
   tCGTGGCCTCACCGAAGAAGGGCTTGGCTGGGTTg                                 >  1:9136589/1‑35 (MQ=255)
    cGTGGCCTCACCGAAGAAGGGCTTGGCTGGgttta                                >  1:10720227/1‑33 (MQ=37)
    cGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGa                                <  1:14821718/35‑1 (MQ=255)
    cGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGa                                <  1:18376837/35‑1 (MQ=255)
    cGTGGCCTCACCGAAGAAGGGCTTGGCTGGGTTgt                                >  1:6364134/1‑34 (MQ=255)
     gTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGAc                               <  1:1496280/35‑1 (MQ=255)
     gTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGAc                               <  1:13324413/35‑1 (MQ=255)
     gTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGAc                               <  1:19711857/35‑1 (MQ=255)
     gTGGCCTCACCGAAGAAGGGCTTGGCTGGGTTgtc                               >  1:2628123/1‑33 (MQ=37)
      tGGCCTCACCGAAGAAGGGCTTGGCTTGTTTGACg                              >  1:11101279/1‑35 (MQ=255)
      tGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACg                              <  1:19465282/35‑1 (MQ=255)
      tGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACg                              <  1:5857845/35‑1 (MQ=255)
      tGGCCTCACCGAAGAAGGGCTTGGCTGGGTTTACg                              >  1:15337238/1‑35 (MQ=25)
      tGGCCTCACCGAAGAAGGGCTTGGCTGGGTTGaag                              >  1:6708529/1‑33 (MQ=37)
       ggCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGc                             <  1:2203199/35‑1 (MQ=255)
        gCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGcc                            <  1:6255908/35‑1 (MQ=255)
         ccTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCt                           <  1:6190788/35‑1 (MQ=255)
         ccTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCt                           <  1:12379090/35‑1 (MQ=255)
         ccTCACCGAAGAAGGGCTTGGCTGGGTTGACGCCt                           >  1:7552402/1‑35 (MQ=255)
          cTCACCGAAGAAGTGCTTGGCTGGTTTGACGCCTg                          >  1:20097899/1‑35 (MQ=255)
          cTCACCGAAGAAGGGCTTGGCTGGGTTTGACGctg                           >  1:17151759/1‑33 (MQ=25)
          cTCACCGAAGAAGGGCTTGGCTGGGTTGACGCCTg                          >  1:4687241/1‑35 (MQ=255)
           tCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTgg                         <  1:17683669/35‑1 (MQ=255)
           tCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTgg                         <  1:1845067/35‑1 (MQ=255)
           tCACCGAAGAAGGGCTTGGCTGGGTTGACGCCttt                         >  1:13394626/1‑33 (MQ=37)
           tCACCGAAGAAGGGCTTGGCTGGGTTGACGCCTgg                         >  1:13341014/1‑35 (MQ=255)
            cACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgc                        <  1:1312113/35‑1 (MQ=255)
            cACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgc                        <  1:6867377/35‑1 (MQ=255)
            cACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgc                        <  1:5662611/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:5507787/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:16692680/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:15870226/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:14325980/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:13641827/35‑1 (MQ=255)
             aCCGAAGAAGGGCTTGGCTGGTTTGACGCCTGgcg                       <  1:2710359/35‑1 (MQ=255)
              ccGAAGAAGGGCTTGGCTGGGTTTTCTCCCGgcgc                      >  1:4059912/1‑35 (MQ=12)
               cGAAGAAGGGCTTGGCTGGGTTGACGCCTGGCGCt                     >  1:6813262/1‑35 (MQ=255)
               cGAAGAAGGGCTTGGCTGGGTTGACGCCCGgggcc                     >  1:16655099/1‑34 (MQ=21)
                gaagaaGGGCTTGGCTGGTTTGACGCCTGGCGCTa                    <  1:560534/35‑1 (MQ=255)
                gaagaaGGGCTTGGCTGGTTTGACGCCTGGCGCTa                    <  1:2339510/35‑1 (MQ=255)
                gaagaaGGGCTTGGCTGGTTTGACGCCTGGCGCTa                    <  1:18804544/35‑1 (MQ=255)
                gaagaaGGGCTTGGCTGGTTTGACGCCTGGCGCTa                    <  1:5104624/35‑1 (MQ=255)
                gaagaaGGGCTTGGCTGGGTTTACGCCCGGCGCtc                    >  1:4422110/1‑34 (MQ=21)
                 aagaagGGCTTGGCTGGTTTGACGCCTGGCGCTaa                   <  1:18109095/35‑1 (MQ=255)
                 aagaagGGCTTGGCTGGTTTGACGCCTGGCGCTaa                   <  1:7779678/35‑1 (MQ=255)
                  agaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAt                  <  1:1413187/35‑1 (MQ=255)
                  agaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAt                  <  1:6105032/35‑1 (MQ=255)
                  agaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAt                  <  1:17923627/35‑1 (MQ=255)
                   gaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAtt                 <  1:11729661/35‑1 (MQ=255)
                   gaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAtt                 <  1:5806816/35‑1 (MQ=255)
                   gaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAtt                 <  1:11441960/35‑1 (MQ=255)
                   gaagGGCTTGGCTGGTTTGACGCCTGGCGCTAAtt                 <  1:8839157/35‑1 (MQ=255)
                   gaagGGCTTGGCTGGTTTGACGCATGGCGCTAAtt                 <  1:15139359/35‑1 (MQ=255)
                   gaagGGCTTAGCTGGTTTGACGCCTGGCGCTAAtt                 <  1:9604737/35‑1 (MQ=255)
                    aagGGCTTGGCTGGTTTGACGCCTGGCGCTAATTc                <  1:17406914/35‑1 (MQ=255)
                    aagGGCTTGGCTGGTTTGACGCCTGGCGCTAATTc                <  1:18725759/35‑1 (MQ=255)
                    aagGGCTTGGCTGGTTTGACGCCTGGCGCTAATTc                <  1:4663837/35‑1 (MQ=255)
                     agGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCg               <  1:15227775/35‑1 (MQ=255)
                     agGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCg               <  1:10052359/35‑1 (MQ=255)
                      gggCTTGGCTGGTTTGACGCCTGGCGCTAATTCGt              <  1:8595273/35‑1 (MQ=255)
                      gggCTTGGCTGGTTTGACGCCTGGCGCTAATTCGt              <  1:3071922/35‑1 (MQ=255)
                      gggCTTGGCTGGTTTGACGCCTGGCGCTAATTCGt              <  1:12130116/35‑1 (MQ=255)
                      gggCTTGGCTGGTTTGACGCCTGGCGCTAATTCGt              <  1:18640230/35‑1 (MQ=255)
                        gCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTa            >  1:10589766/1‑35 (MQ=255)
                        gCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTa            >  1:9311526/1‑35 (MQ=255)
                        gCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTa            >  1:16028445/1‑35 (MQ=255)
                         cTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTaa           <  1:1911840/35‑1 (MQ=255)
                          ttGGCTGGTTTGACGCCTGGCGCTAATTCGTTaaa          <  1:13962403/35‑1 (MQ=255)
                           tGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAAt         >  1:7952623/1‑35 (MQ=255)
                           tGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAAt         <  1:20242300/35‑1 (MQ=255)
                           tGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAAt         <  1:13601067/35‑1 (MQ=255)
                           tGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAAt         <  1:16892262/35‑1 (MQ=255)
                            ggCTGGTTTGACGCCTGGCGCTAATTCGTTAAATc        >  1:3403321/1‑35 (MQ=255)
                             gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATcc       <  1:8053870/35‑1 (MQ=255)
                             gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATcc       <  1:2053280/35‑1 (MQ=255)
                             gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATcc       <  1:12934702/35‑1 (MQ=255)
                             gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATcc       <  1:18278165/35‑1 (MQ=255)
                             gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATcc       >  1:14899555/1‑35 (MQ=255)
                              cTGGTTTGACGCCTGGCGCTAATTCGTTAAATccc      >  1:20401787/1‑35 (MQ=255)
                               tGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCt     >  1:15584822/1‑35 (MQ=255)
                               tGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCt     >  1:6643396/1‑35 (MQ=255)
                                 ggTTTGACGCCTGGCGCTAATTCGTTAAATCCCTa    <  1:386871/35‑1 (MQ=255)
                                  gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTaa   <  1:7020100/35‑1 (MQ=255)
                                  gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTaa   <  1:12662229/35‑1 (MQ=255)
                                  gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTaa   <  1:7272161/35‑1 (MQ=255)
                                  gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTaa   <  1:11935051/35‑1 (MQ=255)
                                  gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTaa   <  1:10155738/35‑1 (MQ=255)
                                   tttGACGCCTGGCGCTAATTCGTTAAATCCCTAAt  <  1:10987858/35‑1 (MQ=255)
                                   tttGACGCCTGGCGCTAATTCGTTAAATCCCTAAt  <  1:2400855/35‑1 (MQ=255)
                                   tttGACGCCTGGCGCTAATTCGTTAAATCCCTAAt  >  1:3701038/1‑35 (MQ=255)
                                   |                                  
ACCTCGTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAAT  >  REL606/2759822‑2759890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.