Please select a file to upload or enter a sequence into the textbox.

FASTA, GenBank, or BioBrick XML format files are supported. BioBrick XML files are available from the iGEM Registry.

Sequence file:

Sequence:

Organism: E. coli E. coli, RecA-

Annotated Regions Only: Only assign rates for hypermutable sites that overlap with annotated regions. Sequence must be annotated.

Citing the EFM Calculator

Please reference the following publication when citing the EFM Calculator:

Benjamin R. Jack, Sean P. Leonard, Dennis M. Mishler, Brian A. Renda, Dacia Leon, Gabriel A. Suárez, and Jeffrey E Barrick (2015). Predicting the genetic stability of engineered DNA sequences with the EFM Calculator. ACS Synthetic Biology. Just Accepted Manuscript.
DOI: 10.1021/acssynbio.5b00068